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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/09/2021 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, E. T. de A.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. |
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Afiliação: |
ELIZABETH TEIXEIRA DE ALMEIDA RAMOS, UFRRJ; CARLOS HENRIQUE SALVINO GADELHA MENESES, UEPB; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Annals of Applied Biology, Annals of Applied Biology, v. 180, p. 163-175. 2022. |
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DOI: |
DOI: 10.1111/aab.12721 |
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Idioma: |
Inglês |
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Conteúdo: |
Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides. MenosGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, th... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial peptide; Bactericidal effect. |
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Thesaurus Nal: |
Biological control; Gluconacetobacter diazotrophicus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02455naa a2200217 a 4500
001 2134566
005 2023-08-28
008 2022 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1111/aab.12721$2DOI
100 1 $aRAMOS, E. T. de A.
245 $aCharacterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans.$h[electronic resource]
260 $c2022
520 $aGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.
650 $aBiological control
650 $aGluconacetobacter diazotrophicus
653 $aAntimicrobial peptide
653 $aBactericidal effect
700 1 $aMENESES, C. H. S. G.
700 1 $aVIDAL, M. S.
700 1 $aBALDANI, J. I.
773 $tAnnals of Applied Biology, Annals of Applied Biology$gv. 180, p. 163-175. 2022.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 111 | |
| 2. |  | FILGUEIRAS, L.; SILVA, R.; VIDAL, M. S.; BALDANI, J. I.; MENESES, C. H. G. de. Application of Gluconacetobacter diazotrophicus increases tolerance of red rice variety to water stress. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 68 Session 6, Poster VI.8| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 8. | | ALLQUERES, S.; MENESES, C.; ROTHBALLER, M.; MARTINS, O.; BALDANI, J. I.; SCHMID, M.; HARTMANN, A. The role of reactive oxygen species during gluconacetobacter diazotrophicus- rice interaction. INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 13., 2012. Abstracts... Neuherberg: German Research Center for Environmental Health, 2012. 28| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 9. | | ROUWS, L. F. M.; MENESES, C. H. G.; GUEDES. H. V.; VIDAL, M. S.; SCHWAB, S. Use of reporter genes to monitor interactions between the diazotrophic bacterium Gluconacetobacter diazotrophicus and its host plants sugarcane and rice. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 12. | | PEREIRA, K.; ALMEIDA, I.; VIDAL, M. S.; BALDANI, J. I.; MENESES, C. H. G. de. Self-regulation of exopolysaccharide production in Gluconacetobacter diazotrophicus by a tyrosine kinase. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 66 Session 6, Poster VI.9| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | SIMÕES-ARAÚJO, J. L. de; SILVA-JÚNIOR, Z.; MENESES, C. H. S. G.; VIDAL, M. S. Expression of VuNIP1 (NOD26-like) in cowpea nodules under water stress. In: ANNUAL MEETING OF SBBq, 37., CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia. Abstracts... Águas de Lindóia: SBBq, 2008. 1 p. Parceria: UFRJ.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 18. | | ROUWS, L. F. M.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. Evidence for an important role of the Gluconacetobacter diazotrophics Flp pilus during rice root surface colonization. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SVI.08| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | ALQUÉRES, S.; MENESES, C. H. G de; ROUWS, L. F. M.; ROTHBALLER, M.; BALDANI, J. I.; SCHMID, M.; HARTMANN, A. The bacterial superoxide dismutase and glutathione reductase are crucial for endophytic colonization of rice roots by Gluconacetobacter diazotrophicus PAL5. Molecular Plant-Microbe Interactions, v. 26, n. 8, p. 937-946, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 111 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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